On-pathway versus off-pathway folding intermediates
نویسندگان
چکیده
منابع مشابه
Absence of stable intermediates on the folding pathway of barnase.
Barnase is one of the few protein models that has been studied extensively for protein folding. Previous studies led to the conclusion that barnase folds through a very stable submillisecond intermediate ( approximately 3 kcal/mol). The structure of this intermediate was characterized intensively by using a protein engineering approach. This intermediate has now been reexamined with three direc...
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Refolding of the very-fast-folding unfolded species (Uvf) of disulfide-intact bovine pancreatic ribonuclease A has been monitored by circular dichroism (CD) at 222 and 275 nm at 0.9 or 2.6 M guanidine hydrochloride, pH 7.0, and 5 degrees C. The refolding of Uvf represents a purely conformational folding process which is not complicated by cis-trans proline isomerization. The data indicate that ...
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The folding of a protein-like heteropolymer is studied by direct simulation of a lattice model that folds rapidly to a well-defined “native” structure. The details of each molecular folding event depend on the random initial conformation as well as the random thermal fluctuations of the polymer. By analysing the statistical properties of hundreds of folding events, a classical folding “pathway”...
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ژورنال
عنوان ژورنال: Folding and Design
سال: 1996
ISSN: 1359-0278
DOI: 10.1016/s1359-0278(96)00003-x